Maternal Expression Relaxes Constraint on Innovation of the Anterior Determinant, bicoid

The origin of evolutionary novelty is believed to involve both positive selection and relaxed developmental constraint. In flies, the redesign of anterior patterning during embryogenesis is a major developmental innovation and the rapidly evolving Hox gene, bicoid (bcd), plays a critical role. We report evidence for relaxation of selective constraint acting on bicoid as a result of its maternal pattern of gene expression. Evolutionary theory predicts 2-fold greater sequence diversity for maternal effect genes than for zygotically expressed genes, because natural selection is only half as effective acting on autosomal genes expressed in one sex as it is on genes expressed in both sexes. We sample an individual from ten populations of Drosophila melanogaster and nine populations of D. simulans for polymorphism in the tandem gene duplicates bcd, which is maternally expressed, and zerknüllt (zen), which is zygotically expressed. In both species, we find the ratio of bcd to zen nucleotide diversity to be two or more in the coding regions but one in the noncoding regions, providing the first quantitative support for the theoretical prediction of relaxed selective constraint on maternal-effect genes resulting from sex-limited expression. Our results suggest that the accelerated rate of evolution observed for bcd is owing, at least partly, to variation generated by relaxed selective constraint

A Total Evidence Approach to Understanding Phylogenetic Relationships and Ecological Diversity in Selanginella subg. Tetragonostachys

• Premise of the Study: Several members of Selaginella are renowned for their ability to survive extreme drought and “resurrect” when conditions improve. Many of these belong to subgenus Tetragonostachys , a group of ~45 species primarily found in North and Central America, with substantial diversity in the Sonoran and Chihuahuan Deserts. We evaluated the monophyly and the age of subgenus Tetragonostachys and assess how drought tolerance contributed to the evolution of this clade. • Methods: Our study included most Tetragonostachys species, using plastid and nuclear sequences, fossil and herbarium records, and climate variables to describe the species diversity, phylogenetic relationships, divergence times, and climatic niche evolution in the subgenus. • Key Results: We found that subgenus Tetragonostachys forms a monophyletic group sister to Selaginella lepidophylla and may have diverged from other Selaginella because of a Gondwanan–Laurasian vicariance event ca. 240 mya. The North American radiation of Tetragonostachys appears to be much more recent and to have occurred during the Early Cretaceous–late Paleocene interval. We identifi ed two signifi cant and nested ecological niche shifts during the evolution of Tetragonostachys associated with extreme drought tolerance and a more recent shift to cold climates. Our analyses suggest that drought tolerance evolved in the warm deserts of southwest North America and may have been advantageous for colonization of cold and dry boreal climates. • Conclusions: Our investigation provides a foundation for future research addressing the genomics of ecological niche evolution and the potential role of reticulate evolution in Selaginella subgenus Tetragonostachys .The authors thank P. Korall for sharing data. S. Buerki, N. Alvarez, and B. Marazzi provided technical assistance and valuable comments on the manuscript. N.A. was funded by the Swiss National Science Foundation (grant no. PBNEP3-132747). C.L.A. was funded by the Swedish Research Council

Why does early childhood deprivation increase the risk for depression and anxiety in adulthood? A developmental cascade model

Abstract Background: Using data from the English & Romanian Adoptees (ERA) study we recently reported that early time-lmited exposure to severe institutional deprivation is associated with early onset and persistent neurodevelopmental problems and later onset emotional problems. Here we examine possible reasons for the late emergence of emotional problems in this cohort. Our main focus is on testing a developmental cascade mediated via the functional impact of early-appearing neurodevelopmental problems on late adolescent functioning. We also explore a second putative pathway via sensitization to stress. Methods: The ERA study includes 165 Romanian individuals who spent their early lives in grossly depriving institutions and were subsequently adopted into UK families, along with 52 UK adoptees with no history of deprivation. Age six years symptoms of neurodevelopmental problems and age 15 anxiety/depression symptoms were assessed via parental reports. Young adult symptoms of depression and anxiety were assessed by both parent and self-reports; young adults also completed measures of stress reactivity , exposure to adverse life events and functioning in work and interpersonal relationships. Results: The path between early institutional deprivation and adult emotional problems was mediated via the impact of early neurodevelopmental problems on unemployment and poor friendship functioning during the transition to adulthood. The findings with regard to early deprivation, later life stress reactivity and emotional problems were inconclusive. Conclusions: Our analysis suggests that the risk for adult depression and anxiety following extreme institutional deprivation is explained through the effects of early neurodevelopmental problems on later social and vocational functioning. Future research should more fully examine the role of stress susceptibility in this model

Alcohol, drug and related health and wellbeing issues among young people completing an online screen.

Objective: Despite high levels of alcohol use, drug use and risky behaviors, rates of help-seeking amongst young people are typically low. This study explored the profile of young people (under the age of 25 years) completing an online screen, assessing substance use problem severity and wellbeing in comparison with adults completing the same screen, so as to inform development of better targeted approaches for this in-need population. Methods: Between 2012 and 2014, an online alcohol and drug screen was promoted across Australia on a national online counseling service. The screen assessed severity of substance use, mental health and wellbeing. Results: A total of 2939 screens were completed between December 2012 and May 2014, with 18% completed by young people. Young people reported a high severity of substance use problems (44% reported likely drug dependence) and reported significantly poorer mental health and wellbeing than adults completing the screen. This suggests that there is a population of young people in need of support who could be initially engaged through online screening. Conclusions: Online screening should be a key component of engagement strategies for adolescent and early adult help-seeking

Support for AAC Use in Preschool, and Growth in Language Skills, for Young Children with Developmental Disabilities

Little is known about how AAC use in preschool may impact language development for children with complex communication needs (e.g., children with autism, cerebral palsy, Down syndrome, and other developmental disabilities). We developed two surveys (a) to describe children’s use of AAC in preschool classrooms, as well as the use of prompts and question asking, and augmented input by their communication partners; and (b) to describe teachers’ experience, training, and perceived support in providing AAC. We then examined the relationship between children’s experience of AAC, including the use of prompts, question asking, and augmented input by their partners, and the growth of receptive and expressive language for 71 children with developmental disabilities over a two-year period. The use of AAC by peers to provide augmented input was associated with stronger language growth; the use of prompting and question asking by teachers was associated with weaker language growth. Teachers reported that they received little training regarding ways to support a child’s use of AAC. Results suggest the need for further research on promoting AAC use at the preschool level, including research to promote peer interactions for AAC users

A tiered model of substance use severity and life complexity : potential for application to needs-based planning

Background: In order to improve long-term outcomes for individuals with substance use problems, one approach is to adopt a system planning model that considers both addiction severity and life complexities. The tiered approach has been developed and tested to describe systems-level need based on levels of risk and problem severity. Methods: An existing tiered model was modified to accommodate Australian data, incorporating substance use severity and life complexity. The hypothesis was that tiers would reflect differences in well-being amongst help seekers such that an increase in tier would be associated with a reduction in well-being, suggesting the need for more intensive (and integrated) interventions. The model was tested using 2 data sets of screening data, collected from face-to-face alcohol and other drug (AOD) service (n = 430) and online help (n = 309) seekers, drawn from a larger sample of 2,766 screens. The screen included demographic information and substance use, mental health, and quality of life measures. Results: There was a significant relationship between well-being and tier ranking, suggesting that the model adequately captured elements of severity and complexity that impact on well-being. There were notable differences between the help-seeking populations with a higher proportion of online respondents allocated to lower tiers and more face-to-face respondents allocated to higher tiers. However, there was an overlap in these populations, with more than half of online respondents classified as higher tiers and one fifth of face-to-face respondents classified as lower tiers. This suggests that the model can be used both to assess unmet need in out-of-treatment groups and demand in the absence of dependence in a subpopulation of the face-to-face treatment population. Conclusions: The tiered model provides a method to understand levels of AOD treatment need and, as part of needs-based planning, may be used to optimize treatment responses and resourcing

Nutritional and Demographic Consequences of Varying Elk Migratory Behaviors

Elk (Cervus elaphus) populations in the American West exhibit wide variation in migratory behavior. The traditional view of elk migration holds that migratory elk move from winter range in order to track growth of highly nutritious fresh vegetation into higher elevation areas. Non-migratory elk forego this seasonal movement, typically foraging in lower elevation winter range areas throughout the summer. Although the effect of summer nutrition on elk body condition and reproductive success is well known, the nutritional and demographic consequences of these differing migratory behaviors remain unclear. We developed a predictive model of summer forage quality to compare the nutrition available to migrants and non-migrants in a partially migratory population of elk in western Montana. Non-migratory elk had access to significantly higher forage quality than their migratory counterparts; the lower forage quality available to migrants is predicted to result in reduced reproductive success based on published studies linking nutrition with elk demographic rates. We therefore expect non-migrants to have higher fecundity rates and to comprise a higher proportion of the population relative to migrants. Harvest management actions that reduce survival rates of non-migrants or increase survival rates of migrants may be an effective tool for maintaining migratory behavior in partially migratory populations

Relativistic Celestial Mechanics with PPN Parameters

Starting from the global parametrized post-Newtonian (PPN) reference system with two PPN parameters $\gamma$ and $\beta$ we consider a space-bounded subsystem of matter and construct a local reference system for that subsystem in which the influence of external masses reduces to tidal effects. Both the metric tensor of the local PPN reference system in the first post-Newtonian approximation as well as the coordinate transformations between the global PPN reference system and the local one are constructed in explicit form. The terms proportional to $\eta=4\beta-\gamma-3$ reflecting a violation of the equivalence principle are discussed in detail. We suggest an empirical definition of multipole moments which are intended to play the same role in PPN celestial mechanics as the Blanchet-Damour moments in General Relativity. Starting with the metric tensor in the local PPN reference system we derive translational equations of motion of a test particle in that system. The translational and rotational equations of motion for center of mass and spin of each of $N$ extended massive bodies possessing arbitrary multipole structure are derived. As an application of the general equations of motion a monopole-spin dipole model is considered and the known PPN equations of motion of mass monopoles with spins are rederived.Comment: 71 page

The Stellar Populations of M33's Outer Regions IV: Inflow History and Chemical Evolution

We have modelled the observed color-magnitude diagram (CMD) at one location in M33's outskirts under the framework of a simple chemical evolution scenario which adopts instantaneous and delayed recycling for the nucleosynthetic products of Type II and Ia supernovae. In this scenario, interstellar gas forms stars at a rate modulated by the Kennicutt-Schmidt relation and gas outflow occurs at a rate proportional to the star formation rate (SFR). With this approach, we put broad constraints on the role of gas flows during this region's evolution and compare its [alpha/Fe] vs. [Fe/H] relation with that of other Local Group systems. We find that models with gas inflow are significantly better than the closed box model at reproducing the observed distribution of stars in the CMD. The best models have a majority of gas inflow taking place in the last 7 Gyr, and relatively little in the last 3 Gyr. These models predict most stars in this region to have [alpha/Fe] ratios lower than the bulk of the Milky Way's halo. The predictions for the present-day SFR, gas mass, and oxygen abundance compare favorably to independent empirical estimates. Our results paint a picture in which M33's outer disc formed from the protracted inflow of gas over several Gyr with at least half of the total inflow occurring since z ~ 1.Comment: 22 pages, 12 figures, accepted to MNRA